Regulatory

Part:BBa_J100090:Design

Designed by: Caroline Vrana   Group: Campbell M Lab   (2012-07-18)

CRISPR sequence with GFP and AmpR targets


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 392
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 393
    Illegal PstI site found at 407
    Illegal NotI site found at 7
    Illegal NotI site found at 400
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 393
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 393
    Illegal PstI site found at 407
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Ampicillin Resistance spacer came from the pSB1A2 plasmid (part J119043) GFP target spacer came from part E0040. Leader sequence came from the 2011 USC iGEM team. The sequence for the CRISPR repeats comes from Touchon M, Rocha EPC (2010) The Small, Slow and Specialized CRISPR and Anti-CRISPR of Escherichia and Salmonella. PLoS ONE 5(6): e11126. doi:10.1371/journal.pone.0011126 and Mojica FJ et. al.Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology. 2009 Mar;155(Pt 3):733-40.

References